ABSTRACT
Objective:To analyze the influencing factors of early miscarriage after in vitro fertilization/intracytoplasmic sperm injection-embryo transfer treatment.Methods:From January 2015 to August 2018, 461 cases of pregnant women who received mid luteal phase ovulation induction in Beijing Obstetrics and Gynecology Hospital, Capital Medical University were retrospectively analyzed.According to the pregnancy outcome, the patients were divided into early miscarriage group (54 cases) and delivery group (407 cases). The general situation and ovulation induction outcome of the two groups were compared, and the related factors of early spontaneous miscarriage were analyzed.Results:There was no significant difference in age, pre pregnancy body mass index, type of infertility, history of abortion, thyrotropin, basal endocrine level, number of antral follicles, daily hormone level of hCG injection, and number of oocytes retrieved between early miscarriage group and delivery group (all P>0.05). Logistic regression analysis showed that age and basal follicle stimulating hormone were risk factors for early miscarriage.The risk of early miscarriage in patients with age ≥ 35 years old was 1.854 times higher than that in patients younger than 35 years old, 95% CI 1.027-3.347, P=0.041; the risk of early abortion in patients with basal FSH ≥ 10 U/L was 1.499 times higher than that in patients with basal FSH<10 U/L, 95% CI 0.776-2.895, P=0.048. Conclusion:Age and basal follicle stimulating hormone are risk factors for early miscarriage after long protocol assisted pregnancy in mid luteal phase.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of anti-apoptosis gene (bcl-X(L)), cytochrome c and caspase-3 activity on chemoresistance in cisplatin-resistant human ovarian cancer cell lines (A2780/DDP, COC1/DDP).</p><p><b>METHODS</b>The expression of bcl-X(L) cisplatin treated cytochrome c and caspase-3 activity were monitored by RT-PCR and Western blot in cisplatin-resistant (A2780/DDP, COC1/DDP) and cisplatin-sensitive (A2780, COC1) cell lines. The apoptotic rates of A2780, COC1, A2780/DDP and COC1/DDP were detected with flow cytometry after having been treated by cisplatin.</p><p><b>RESULTS</b>The expression of bcl-X(L) in A2780/DDP and COC1/DDP was significantly higher than that in A2780 and COC1 cells, whereas the expression of cytochrome c, caspase-3 activity and apoptotic rates of A2780/DDP and COC1/DDP were significantly reduced more than those of A2780 and COC1 after having been treated by cisplatin (P < 0.05).</p><p><b>CONCLUSION</b>The overexpression of anti-apoptotic gene bcl-X(L), which downregulates cytochrome c and decreases caspase-3 activity, may be related to cisplatin-resistance in human ovarian cancer cell lines.</p>